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Regulation of transcription of the cell gene in Agaricus bisporus
Author(s) -
Yagüe Ernesto,
Wood David A.,
Thurston Christopher F.
Publication year - 1994
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1994.tb00993.x
Subject(s) - agaricus bisporus , biology , gene , transcription (linguistics) , mycelium , cellulase , gene expression , microbiology and biotechnology , cellulose , biochemistry , mushroom , botany , linguistics , philosophy
Summary Northern analysis showed that accumulation of Agaricus bisporus cell mRNA was regulated by two independent mechanisms: (i) induction by cellulose; and (ii) repression by glucose and other sugars, Isolated A. bisporus nuclei were transcriptionally active. Nuclei Isolated from cellulose‐grown mycelium synthesized six times more cell mRNA than nuclei from glucose‐grown mycelium. The start point of transcription ( tsp ) was identified by primer extension and S1 nuclease analysis. Putative glucose‐, and cAMP‐responsive elements as well as regions with homology to promoter regions of other fungal cellulase genes were detected both upstream and downstream from the tsp of the cel1 gene.