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Regulation of R100 conjugation requires traM in cis to traJ
Author(s) -
Dempsey Walter B.
Publication year - 1994
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1994.tb00490.x
Subject(s) - biology , operon , complementation , open reading frame , mutant , gene , genetics , promoter , lac operon , microbiology and biotechnology , plasmid , gene expression , peptide sequence
Summary Deletion mutants of R100‐1 were constructed by classical methods to remove various segments of the traM open reading frame, pTraM‐binding sites and the traM promoters. Complementation tests showed that traM was efficiently complemented only when the frans‐acting fragment contained both the complete traM gene and the adjacent traJ promoter and leader sequences. The conclusion is that traM and traJ constitute a complex operon. A deletion mutant lacking all of the fraJ gene, and one containing a frameshifting traM deletion, retained the ability to transfer at a low level, thereby showing that neither pTraM nor pTraJ is absolutely essential for transfer.