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Neither absence nor excess of λ O initiator‐digesting ClpXP protease affects λ plasmid or phage replication in Escherichia coli
Author(s) -
Szalewska Agnieszka,
Wȩgrzyn Grzegorz,
Taylor Karol
Publication year - 1994
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1994.tb00441.x
Subject(s) - biology , dnaa , escherichia coli , plasmid , lambda phage , protease , lambda , dna replication , bacteriophage , dna , microbiology and biotechnology , rolling circle replication , proteolysis , genetics , origin of replication , biochemistry , enzyme , gene , physics , optics
Summary Owing to rapid proteolysis of the coliphage λ‐coded initiator protein, λ O, this protein is considered to carry a rate–limiting step in λ DNA replication. The discovery of ClpXP protease responsible for λ O protein turnover allowed an opportunity to verify this hypothesis. However, neither absence nor excess of this protease significantly affected the transformation efficiency and copy number of λ plasmid, or the Kinetics of the λ phage growth. These results are also incompatible with the hypothesis that the stabilization of λ O plays a role in the switch from early (circle‐to‐circle) to late (rolling‐circle) λ phage DNA replication. Tran‐scriptional activation of oriλ probably assisted by the Escherichia coli DnaA function, remains as the possible rate‐limiting step in λ DNA replication.