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Cloning of the nupC gene of Escherichia coli encoding a nucleoside transport system, and identification of an adjacent Insertion element, IS 186
Author(s) -
Craig Jane E.,
Zhang Yianbiao,
Gallagher Maurice P.
Publication year - 1994
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1994.tb00392.x
Subject(s) - biology , escherichia coli , open reading frame , gene , homology (biology) , terminator (solar) , genetics , microbiology and biotechnology , peptide sequence , ionosphere , physics , astronomy
Summary Escherichia coli is known to contain more than one active transport system for nucleoside uptake. In the present study we report the sequence of a gene encoding a second nucleoside transport system, nupC (in addition to nupG. ) An open reading frame (ORF) of 1200bp was identified that codes for a hydrophobic polypeptide of 43 560 Da and an NupC fusion protein was shown to be membrane associated. The native NupC protein is also identified, following over‐expression. NupC exhibits short regions of homology to several membrane‐associated proteins, including LacY and Cyd. Analysis of the nupC promoter region revealed the presence of at least two putative CRP‐binding sites, centred at–40bp and–89bp, which probably flank a CytR‐binding site. In addition, an adjacent IS186 element was identified and found to reside within a putative terminator structure, downstream from the nupC ORF. This arrangement is shown to reflect the previously established gene order on the E. coli chromosome.