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Entamoeba histolytica: electron‐dense granule secretion, collagenase activity and virulence are altered in the cytoskeleton mutant BG‐3
Author(s) -
Jesús Serrano José,
Garza Mireya,
Moreno Miguel Angel,
Tovar Rosalinda,
León Gloria,
Tsutsumi Victor,
Lourdes Muñoz Maria
Publication year - 1994
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1994.tb00356.x
Subject(s) - entamoeba histolytica , secretion , virulence , biology , mutant , granule (geology) , microbiology and biotechnology , collagenase , dense granule , cytochalasin d , cytochalasin , in vivo , cytoskeleton , cell , immunology , biochemistry , enzyme , gene , genetics , paleontology , platelet , platelet activation
Summary HM‐1:IMSS, a pathogenic strain of Entamoeba histolytica , and its mutant BG‐3, Identified by resistance to cytochalasin D, were tested for their capacity to: (i) secrete electron‐dense granules; (ii) adhere and digest native type I collagen gels; and (iii) produce liver abscesses in new‐born hamsters. The results demonstrate that the mutant has low adherence to collagen, low electron‐dense granule secretion and collagenolytic activity, and low capacity to produce liver lesions in vivo , compared with the parental strain HM1:IMSS.