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Isolation and characterization of IS 31831, a transposable element from Corynebacterium glutamicum
Author(s) -
Vertès Alain A.,
Inui Masayuki,
Kobayashi Miki,
Kurusu Yasurou,
Yukawa Hideaki
Publication year - 1994
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1994.tb00351.x
Subject(s) - corynebacterium glutamicum , transposable element , biology , corynebacterium , bacillus subtilis , insertion sequence , shuttle vector , transposase , genetics , bacteria , escherichia coli , corynebacterium diphtheriae , microbiology and biotechnology , plasmid , genome , gene , vector (molecular biology) , recombinant dna , virology , vaccination , diphtheria
Summary A transposable element from a coryneform bacterium, Corynebacterium glutamicum ATCC 31831 was isolated and characterized. The element IS 31831 is a 1453 bp insertion sequence with 24 bp imperfect terminal inverted repeats. It contains one open reading frame highly homologous at the amino acid level to the transposase of IS 1096 from Mycobacterium smeg‐matis. Both IS 31831 and IS 1096 exhibit several common characteristics suggesting that they constitute a new family of insertion sequences. IS 31831 was isolated by taking advantage of the sucrose sensitivity of coryneform bacteria conferred by expression of the Bacillus subtilis sacB gene. An Escherichia coli/ Corynebacterium shuttle vector useful for the isolation of transposable elements from the coryneform group of bacteria was constructed.