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Xcp‐mediated protein secretion in Pseudomonas aeruginosa : identification of two additional genes and evidence for regulation of xcp gene expression
Author(s) -
Akrim Mohammed,
Bally Marc,
Ball Geneviève,
Tommassen Jan,
Teerink Henk,
Filloux Alain,
Lazdunski Andrée
Publication year - 1993
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1993.tb02674.x
Subject(s) - biology , operon , gene , signal peptide , bacterial outer membrane , secretion , genetics , pathogenicity island , klebsiella oxytoca , peptide sequence , escherichia coli , biochemistry , enterobacteriaceae
Summary In Pseudomonas aeruginosa , several exoproteins synthesized with a signal sequence (elastase, lipase, phospholipases, alkaline phosphatase and exotoxin A) are secreted by a two‐step mechanism. They first cross the inner membrane in a signal sequence‐dependent way, and are further translocated across the outer membrane in a second step requiring secretion functions encoded by several xcp genes. Ten xcp genes have already been characterized (Bally et al. , 1992a). In this study, two additional xcp genes, xcpP and xcpQ , are described. They are located in the 40 min region of the chromosome where they probably define an operon, divergent from the xcpR–Z operon previously characterized in this region. These two genes encode two proteins, XcpP and XcpQ, similar to PulC and PulD of the pul system of Klebsiella oxytoca. Moreover, the two divergent operons share a common regulation which is growth‐phase dependent.