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Phosphorylation of Spo0A activates its stimulation of in vitro transcription from the Bacillus subtilis spollG operon
Author(s) -
Bird T. H.,
Grimsiey J. K.,
Hoch J. A.,
Spiegelman G. B.
Publication year - 1993
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1993.tb01734.x
Subject(s) - biology , operon , bacillus subtilis , transcription (linguistics) , microbiology and biotechnology , phosphorylation , in vitro , stimulation , gal operon , transcription factor , genetics , bacteria , gene , mutant , neuroscience , linguistics , philosophy
Summary The spollG operon of Bacillus subtilis codes for a sporulation‐specjfic sigma factor, σ; E , In vivo expression of the spollG promoter is activated shortly after the onset of sporulation and is dependent on kinA, spo0F, spo0B and spo0A genes. The products of these genes have been shown to participate in a phosphorelay reaction in vitro , culminating in phosphorylation of the transcription factor, Spo0A. The effect of Spo0A phosphorylation on in vitro transcription from the spollG promoter was determined. Aliquots from phos‐phorelay reactions enhanced spollG promoter activity 10‐fold in transcription assays and stimulation of transcription was dependent on Spo0A phosphorylation. Our results provide biochemical evidence that Spo0A and the phosphoreiay form a signal transduction pathway which activates spoil gene expression in development.