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The partition ( par ) locus of pSC101 is an enhancer of plasmid incompatibility
Author(s) -
Miller Christine A.,
Cohen Stanley N.
Publication year - 1993
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1993.tb01730.x
Subject(s) - plasmid , biology , t dna binary system , genetics , puc19 , locus (genetics) , origin of replication , enhancer , dna , gene , vector (molecular biology) , recombinant dna , transcription factor
Summary The incompatibitity that pSC101‐derived plasmids express toward each other is mediated by directly repeated sequences (iterons) located near the plasmid's replication origin. We report here that the pSC101 par locus, which stabilizes plasmid inheritance in dividing cell populations and alters DNA superheliclty, can function as a cis‐acting enhancer of incompatibility, which we show is determined jointly by the copy number of the plasmid and the number of iterons per copy. A single synthetic 32 bp iteron sequence carried by the pUC19 plasmid confers strong pSC101‐specific incompatibility in the absence of any other pSC101 sites but requires the par locus to express strong incompatibility when carried by a lower‐copy‐number plasmid. We propose a model by which the par locus can enchance the apparently antagonistic processes of incompatibility and pSC101 DNA replication while concurrently facilitating plasmid distribution during cell division.