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A family of IS 1031 elements in the genome of Acetobacter xylinum : nucleotide sequences and strain distribution
Author(s) -
Coucheron Dag H.
Publication year - 1993
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1993.tb01682.x
Subject(s) - biology , nucleic acid sequence , genetics , nucleotide , open reading frame , genome , direct repeat , insertion sequence , strain (injury) , sequence (biology) , dna , microbiology and biotechnology , gene , peptide sequence , transposable element , anatomy
Summary An insertion sequence (here called IS 1031A) from Acetobacter xylinum ATCC 23769 has recently been isolated. This study describes the complete nucleotide sequence of IS 1031A as well as the sequences of two novel iso‐IS 1031 elements, IS1031C and IS1031D, from A. xylinum ATCC 23769. The three ISs are all exactly 930 bp long, have imperfect terminal inverted repeats of 24 bp for IS1031A and 21 bp for IS1031C and IS1031D, are flanked by three base pair direct repeats, and contain an open reading frame encoding a putative basic protein of 278 amino acids. Because of nucleotide substitutions, IS1031C and IS1031D differ from IS 1031A by 12.9% while IS1031C differs from IS1031D by only 0.6%. Hybridization analyses of total DNA from nine A. xylinum strains showed that all strains contained IS 1031‐like elements varying in copy number from three to at least 16. None of three Acetobacter aceti strains examined contained IS1031‐like elements. Taken together, the results suggest that A. xylinum contains a family of IS 1031 elements with considerably diversified nucleotide sequences.