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Cloning and nucleotide sequence of anaerobically induced porin protein E1 (OprE) of Pseudomonas aeruginosa PAO1
Author(s) -
Yamano Yoshinori,
Nishikawa Tohru,
Komatsu Yoshihide
Publication year - 1993
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1993.tb01643.x
Subject(s) - porin , biology , nucleic acid sequence , peptide sequence , amino acid , biochemistry , nucleotide , pseudomonas aeruginosa , microbiology and biotechnology , gene , signal peptide , bacterial outer membrane , genetics , escherichia coli , bacteria
Summary The porin oprE gene of Pseudomonas aeruginosa PAO1 was isolated. Its nucleotide sequence indicated that the structural gene of 1383 nucleotide residues encodes a precursor consisting of 460 amino acid residues with a signal peptide of 29 amino acid residues, which was confirmed by the N ‐terminal 23‐amino‐acid sequence and the reaction with anti‐OprE polyclonal antiserum. Anaerobiosis induced OprE production at the transcription level. The transcription start site was determined to be 40 nucleotides upstream from the ATG initiation codon. The control region contained an appropriately situated Eσ 54 recognition site and the putative second half of an ANR box. The amino acid sequence of OprE had some clusters of sequence homologous with that of OprD of P. aeruginosa , which might be responsible for the outer membrane permeability of imipenem and basic amino acids.