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Transformation with katG restores isoniazid‐sensitivity in Mycobacterium tuberculosis isolates resistant to a range of drug concentrations
Author(s) -
Zhang Ying,
Garbe Thomas,
Young Douglas
Publication year - 1993
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1993.tb01596.x
Subject(s) - biology , isoniazid , mycobacterium tuberculosis , transformation (genetics) , virology , tuberculosis , microbiology and biotechnology , drug resistance , genetics , medicine , pathology , gene
Summary Isoniazid‐resistant isolates of Mycobacterium tuberculosis were transformed with a plasmid vector carrying the functional catalase‐peroxidase ( katG ) gene. Expression of katG restored full drug susceptibility in isolates initially resistant to concentrations ranging from 3.2 to >50μgml −1 . Transformation with the corresponding katG gene from Escherichia coli resulted in low‐level expression of catalase and peroxidase activities and conferred partial isoniazid sensitivity.