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Molecular basis of streptomycin resistance in Mycobacterium tuberculosis : alterations of the ribosomal protein S12 gene and point mutations within a functional 16S ribosomal RNA pseudoknot
Author(s) -
Finken Marion,
Kirschner Philip,
Meier Albrecht,
Wrede Annette,
Böttger Erik C.
Publication year - 1993
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1993.tb01253.x
Subject(s) - biology , pseudoknot , ribosomal protein , ribosomal rna , gene , mycobacterium tuberculosis , point mutation , genetics , microbiology and biotechnology , streptomycin , 16s ribosomal rna , rna , computational biology , ribosome , mutation , tuberculosis , antibiotics , medicine , pathology
Summary Multidrug‐resistant strains of Mycobacterium tuberculosis have resulted in several recent outbreaks. Recognition of drug resistance is important both for treatment and to prevent further transmission. Here we use molecular biology techniques to study the basis of streptomycin resistance in single and multi‐drug‐resistant M. tuberculosis. We demonstrate that streptomycin resistance is associated with mutations implicated in ribosomal resistance. The mutations found either lead to amino acid changes in ribosomal protein SI2 or alter the primary structure of the 16S rRNA. The 16S rRNA region mutated perturbs a pseudoknot structure in a region which has been linked to ribosomal S12 protein.