Premium
High‐resolution mapping of genetic loci of Anabaena PCC 7120 required for photosynthesis and nitrogen fixation
Author(s) -
Kuritz Tanya,
Ernst Anneliese,
Black Todd A.,
Wolk C. Peter
Publication year - 1993
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1993.tb01207.x
Subject(s) - biology , genetics , restriction map , gene , genome , restriction enzyme , transposable element , gene mapping , restriction fragment , restriction fragment length polymorphism , fixation (population genetics) , chromosome , plasmid , genotype
Summary A physical map of the Anabaena genome permitted the localization of its genes to chromosomal fragments generated by rarely cutting restriction endonucleases and separated by pulsed‐field gel electrophoresis. We introduce a novel means of mapping more precisely to c. 20 kb by use of rare restriction sites within vectors bearing cloned sequences that undergo homologous recombination with the genome. We thereby localize and orient genes encoding principal photosynthetic pigments. The relative spacing of loci within a single restriction fragment was determined with even higher resolution, as illustrated for genes required for heterocyst development and nitrogen fixation that were marked with transposons. Small, newly visualized restriction fragments of the chromosome were also mapped.