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The Bacillus subtilis addAB genes are fully functional in Escherichia coli
Author(s) -
Kooistra Jan,
Haijema Bert Jan,
Venema Gerard
Publication year - 1993
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1993.tb01182.x
Subject(s) - recbcd , bacillus subtilis , biology , escherichia coli , exonuclease , helicase , lambda phage , plasmid , mutant , dna , microbiology and biotechnology , gene , biochemistry , genetics , bacteria , bacteriophage , rna , dna polymerase
Summary An Escherichia coli recBCD deletion mutant was transformed with plasmids containing the Bacillus subtilis add genes. The transformants had relatively high ATP‐dependent exonuclease‐ and ATP‐dependent helicase activities, and their viability, the ability to repair u.v.‐damaged DNA and the recombination in conjugation were nearly completely restored. The B. subtilis Add enzyme did not show Chi‐activity in phage lambda recombination. The individual B. subtilis Add proteins were not able to form an enzymatically active complex with the E. coli RecB,C,D proteins, and they could not complement the recB,C,D deficiency. Evidence is presented that only two subunits are involved in the B. subtilis ATP‐dependent exonuclease. This is in contrast to E. coli in which the RecBCD enzyme consists of three subunits.