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Organization of Lrp‐binding sites upstream of ilvlH in Salmonella typhimurium
Author(s) -
Wang Qing,
Sacco Margherita,
Ricca Ezio,
Lago Carmine T.,
DeFelice Maurilio,
Calvo Joseph M.
Publication year - 1993
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1993.tb01179.x
Subject(s) - biology , escherichia coli , footprinting , operon , salmonella , dna footprinting , dna , enterobacteriaceae , binding site , microbiology and biotechnology , dna binding protein , genetics , gene , bacteria , base sequence , transcription factor
Summary Lrp, a major regulatory protein in Escherichia coli , controls the expression of numerous operons, including ilvlH. Lrp binds to six sites upstream of ilvlH , and Lrp binding is required for ilvlH expression. We show here that an Lrp‐like protein is also present in Salmonella typhimurium. This protein can bind both E. coli and S. typhimurium ilvlH DNA, as can E. coli Lrp. Methidiumpropyl‐EDTA footprinting studies were performed with purified E. coli Lrp and S. typhimurium ilvlH DNA. Six binding sites were defined, three of them being similar to corresponding sites in E. coli , and three being organized differently. A consensus derived from six S. typhimurium sites is compatible with that derived from a similar analysis of E. coli sequences.