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Molecular analysis of a cytotoxin‐converting phage, φCTX, of Pseudomonas aeruginosa : structure of the attP–cos–ctx region and integration into the serine tRNA gene
Author(s) -
Hayashi Tetsuya,
Matsumoto Hideki,
Ohnishi Makoto,
Terawaki Yoshiro
Publication year - 1993
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1993.tb01157.x
Subject(s) - biology , pseudomonas aeruginosa , serine , gene , transfer rna , microbiology and biotechnology , genetics , pseudomonadales , bacteria , rna , phosphorylation
Summary The Pseudomonas aeruginosa ctx gene encoding cytotoxin is carried by a temperate phage φCTX. The genome of φCTX is a 35.5 kb double‐stranded DNA with cohesive ends ( cos ). It is unique in that the ctx gene and attP site of φCTX exist very close to the respective cohesive ends. In this study, we determined the structure of this attP–cos–ctx region. The termini of φCTX are 21‐base 5′ extended‐single‐stranded DNAs. The ctx gene is located 361 bp downstream of the left end ( cosL ). The attP core sequence of 30 bp exists only 647 bp apart from the right end ( cosR ). The attP–cos–ctx region contains six kinds of repeats and integration host factor‐binding sequences and showed sequence‐directed static bends, suggesting its potential to form a highly ordered structure. In addition, φCTX was found to integrate into the serine tRNA gene which was mapped to the 43–45 min region on the P. aeruginosa chromosome.