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Identification and characterization of IS 1138 , a transposable element from Mycoplasma pulmonis that belongs to the IS3 family
Author(s) -
Bhugra B.,
Dybvig K.
Publication year - 1993
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1993.tb01148.x
Subject(s) - transposable element , biology , identification (biology) , mycoplasma , genetics , characterization (materials science) , element (criminal law) , computational biology , microbiology and biotechnology , genome , botany , gene , physics , law , political science , optics
Summary Insertion sequence (IS) elements are mobile genetic elements found in prokaryotes. We have identified a repetitive element from Mycoplasma pulmonis , a murine pathogen, that is similar to eubacterial IS elements. By subcloning a single strain of M. pulmonis , we isolated a variant clone in which the IS element had undergone an apparent transposition event. The nucleotide sequences of the element, designated IS 1138 , and the target site into which it inserted were determined. IS 1138 consists of 1288bp with 18bp perfect terminal inverted repeats. Sequence analysis of the target site before and after insertion of IS 1138 identified a 3bp duplication of target DNA flanking the element. The predicted amino acids encoded by the major open reading frame of IS 1138 share significant similarity with the transposases of the IS3 family. Southern hybridization analysis indicates that repetitive sequences similar to IS 1138 are present in most, if not all, strains of M. pulmonis , but Is 1138 –like sequences were not detected in other mycoplasmal species.