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Repair of cyclobutane pyrimidine dimers and 6‐4 photoproducts in the fission yeast Schizosaccharomyces pombe
Author(s) -
McCready Shirley,
Carr Antony M.,
Lehmann Alan R.
Publication year - 1993
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1993.tb00959.x
Subject(s) - schizosaccharomyces pombe , pyrimidine dimer , biology , schizosaccharomyces , mutant , saccharomyces cerevisiae , dna repair , wild type , yeast , nucleotide excision repair , genetics , phenotype , mutation , cyclobutane , gene , biochemistry , chemistry , ring (chemistry) , organic chemistry
Summary We have measured repair of both of the major lesions induced by ultraviolet irradiation (cyclobutane pyrimidine dimers and 6‐4 photoproducts) in wild‐type Schizosaccharomyces pombe and in selected rad mutants, including mutants with deletions in genes from the main phenotypic groups. We find that rad13Δ, rad15 and rad16Δ , which are the S. pombe homologues of the excision‐defective Saccharomyces cerevisiae rad2, rad3 and rad1 , respectively, repair lesions somewhat more slowly than the wild type, but still have considerable repair capacity. rad2Δ , also a presumed excision‐defective mutant, behaves similarly. radS and rad9δ , which belong to different phenotypic groups, repair lesions at the same rate as wild‐type cells. These findings provide new evidence that S. pombe has a second repair system for removing ultraviolet damage, which is absent in S. cerevisiae. Surprisingly, this second mechanism repairs lesions very efficiently; its possible nature is discussed.