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Mode and origin of replication of pSAM2, a conjugative integrating element of Streptomyces ambofaciens
Author(s) -
Hagège Juliette,
Pernodet JeanLuc,
Mann Annick Fried,
Guérineau Michel
Publication year - 1993
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1993.tb00950.x
Subject(s) - biology , replication (statistics) , genetics , element (criminal law) , computational biology , streptomyces , microbiology and biotechnology , bacteria , virology , political science , law
Summary pSAM2 is an 11 kb integrating element from Streptomyces ambofaciens that is capable of replication. It generates single‐stranded DNA during replication, and is therefore the first Streptomyces integrating element to be described that may belong to the family of elements, called the ssDNA elements, that replicate by a rolling‐circle mechanism. The direction of replication has been identified. The plus origin ( ori ) of replication and minus origin (M‐O) have been located. Streptomyces lividans harbouring replicating pSAM2 also contain numerous small covalently closed circular DNA molecules (scm) derived from pSAM2. These scm contain ori and extend on both sides of the putative nick site. Sequences at the junction points of these scm are heterogeneous but short direct repeats were always found in the vicinity of these junctions.