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Interactions between the Escherichia coli cyclic AMP receptor protein and RNA polymerase at Class II promoters
Author(s) -
West David,
Williams Roy,
Rhodius Virgil,
Bell Andrew,
Sharma Naveen,
Zou Chao,
Fujita Nobuyuki,
Ishihama Akira,
Busby Stephen
Publication year - 1993
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1993.tb00949.x
Subject(s) - promoter , biology , rna polymerase , transcription (linguistics) , microbiology and biotechnology , rna polymerase ii , camp receptor protein , polymerase , genetics , escherichia coli , biochemistry , dna , gene , gene expression , linguistics , philosophy
Summary The effects of a number of mutations in crp have been measured at different cyclic AMP receptor protein (CRP)‐dependent Class II promoters, where the CRP‐binding site is centred around 411/2 base pairs upstream from the transcription start point. The amino acid substitutions HL159 and TA158 result in reduced CRP‐dependent activation, but the reduction varies from one Class II promoter to another. Deletions in the C ‐terminus of the RNA polymerase alpha subunit suppress the effects of HL159 and TA158. The role of the C ‐terminus of alpha at these promoters is assessed. Other changes at E58, K52 and E96 affect CRP activity specifically at Class II promoters and their role is discussed.