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Large genomic rearrangements of the unstable region in Streptomyces ambofaciens are associated with major changes in global gene expression
Author(s) -
Dary A.,
Kaiser P.,
Bourget N.,
Thompson C. J.,
Simonet J. M.,
Decaris B.
Publication year - 1993
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1993.tb00946.x
Subject(s) - biology , gene , genetics , strain (injury) , genome , gene expression , gene duplication , streptomyces , chromosome , mutant , microbiology and biotechnology , bacteria , anatomy
Summary Global gene expression is dramatically altered by genomic rearrangements in Streptomyces ambofaciens RP181110. Partial genome mapping of two derivatives of strain RP181110 (strains NSA205 and NSA228) revealed rearrangements located in the unstable region of the genome (deletion in strain NSA228; deletion and amplification in strain NSA205). Computerized comparisons of pulse‐labelled proteins separated by two‐dimensional electrophoresis have revealed numerous differences in gene expression among the three strains during both exponential and stationary phases of growth: 31 proteins were absent in both mutant strains, 16 were absent only in strain NSA228, 17 were absent only in strain NSA205 and 9 were found to be present or over expressed in strain NSA205. Thus, in spite of the scarcity of genetic markers in the unstable region and its dispensability for growth under laboratory conditions, these results suggest that it includes genes which are actively expressed. Spontaneous gene amplifications, which occur frequently in this region of the chromosome, can further activate their expression.