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Identification of a gene required for the oxygen‐regulated formation of the photosynthetic apparatus of Rhodobacter capsulatus
Author(s) -
Pollich Michael,
Jock Susanne,
Klug Gabriele
Publication year - 1993
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1993.tb00945.x
Subject(s) - rhodobacter , biology , open reading frame , operon , mutant , gene , oxygen tension , rhodospirillaceae , microbiology and biotechnology , wild type , rhodospirillales , homology (biology) , genetics , oxygen , peptide sequence , chemistry , organic chemistry
Summary The pigment‐binding proteins of Rhodobacter capsulatus are encoded by the polycistronic puf and puc operons. Both operons show higher expression under low oxygen tension than under high oxygen tension in the wild‐type strain. The Tn5 mutant strain AH2 shows only low levels of puf and puc mRNA under high and low oxygen tension, indicating that it lacks a gene product required for stimulation of puf and puc gene expression under low oxygen tension. The formation of wild‐type levels of photosynthetic complexes and normal oxygen regulation could be restored by the expression in trans of a 1.7 kb fragment of the R. capsulatus wild‐type chromosome or by addition of 10μg I ‐1 vitamin B 12 to the growth medium. An open reading frame of 798 nucleotides containing the Tn5 insertion was identified on the 1.7kb fragment. This open reading frame shows no homology to known genes and has a remarkably high GC content of 76%.