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Transcriptional organization of the Escherichia coli pilus adhesin K99
Author(s) -
Inoue Olivia J.,
Lee John H.,
Isaacson Richard E.
Publication year - 1993
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1993.tb00932.x
Subject(s) - biology , pilus , complementation , operon , bacterial adhesin , catabolite repression , escherichia coli , lac operon , mutant , microbiology and biotechnology , genetics , gene
Summary The production of the Escherichia coli pilus adhesin K99 requires the expression of eight unique proteins: FanA‐H. The transcriptional organization of the K99 operon was investigated by Northern blot analysis. Four RNAs of 0.54, 1.4, 2.5 and 3.5kb were detected. When a fanC probe was used all four RNAs were detected while the use of fanD, fanF and fanG probes detected two RNAs each. Using several deletion and Tn phoA insertion mutants it was concluded that there were seven unique K99‐specific transcripts, several of which were of the same approximate sizes (1.4 and 2.5 kb). It also was concluded that K99 was comprised of at least three complementation groups, two of which were regulated by catabolite repression.