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A proline‐rich protein, verprolin, involved in cytoskeletal organization and cellular growth in the yeast Saccharomyces cerevisiae
Author(s) -
Donnelly Sean F. H.,
Pocklington Michael J.,
Pallotta Dominick,
Orr Elisha
Publication year - 1993
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1993.tb00930.x
Subject(s) - biology , saccharomyces cerevisiae , mutant , yeast , actin , biochemistry , cytoskeleton , homology (biology) , sh3 domain , complementary dna , microbiology and biotechnology , genetics , gene , cell , proto oncogene tyrosine protein kinase src , signal transduction
Summary A gene ( VRP1 ) encoding a novel proline‐rich protein (verprolin) has been isolated from the yeast Saccharomyces cerevisiae as a result of its hybridization to a chick vinculin cDNA probe. The deduced protein sequence contains 24% proline residues present as proline‐rich motifs throughout the verprolin sequence. Several of these motifs resemble recently identified sequences shown to bind Src homology 3 (SH3) domains in vitro. Replacement of the wild‐type VRP1 allele with a mutant allele results in strains that grow slower than wild‐type strains and are temperature sensitive. The vrp1 mutants are impaired in both cell shape and size and display aberrant chitin and actin localization. We propose that verprolin is involved in the maintenance of the yeast actin cytoskeleton, through interactions with other proteins, possibly containing SH3 domains.