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A 3′ transcriptional enhancer within the coding sequence of a yeast gene encoding the common subunit of two multi‐enzyme complexes
Author(s) -
Zaman Z.,
Brown A. J. P.,
Dawes I. W.
Publication year - 1992
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1992.tb02005.x
Subject(s) - biology , gene , enhancer , ura3 , coding region , lac operon , reporter gene , locus (genetics) , microbiology and biotechnology , genetics , gene expression , saccharomyces cerevisiae
Summary A well‐defined set of isogenic yeast strains has been constructed whereby each strain contains a different LPD::lacZ gene fusion integrated at the ura3 locus. These LPD::lacZ fusions differ in the amount of the LPD1 gene (encoding lipoamide dehydrogenase) that is fused to the lacZ reporter. Comparison of the β‐galactosidase activities of each strain during growth on glucose or ethanol revealed that some part of the LPD1 coding region between +13 and +700 is involved in activating gene expression in a carbon source‐dependent manner. This activation occurs at the mRNA level, and is not mediated by changes in mRNA stability. Therefore, the LPD1 gene appears to contain a transcriptional enhancer that lies 3′ to the transcriptional start site, and which responds to carbon source.