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Translation initiation in Escherichia coli : sequences within the ribosome‐binding site
Author(s) -
Ringquist Steven,
Shinedling Sidney,
Barrick Doug,
Green Louis,
Binkley Jonathan,
Stormo Gary D.,
Gold Larry
Publication year - 1992
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1992.tb01561.x
Subject(s) - start codon , biology , shine dalgarno sequence , eukaryotic translation , translation (biology) , ribosomal binding site , ribosome , sequence (biology) , genetics , eukaryotic initiation factor , translational regulation , messenger rna , gene , rna
Summary The translational roles of the Shine‐Dalgarno sequence, the initiation codon, the space between them, and the second codon have been studied. The Shine Dalgarno sequence UAAGGAGG initiated translation roughly four times more efficiently than did the shorter AAGGA sequence. Each Shine‐Dalgarno sequence required a minimum distance to the initiation codon in order to drive translation; spacing, however, could be rather long. Initiation at AUG was more efficient than at GUG or UUG at each spacing examined; initiation at GUG was only slightly better than UUG. Translation was also affected by residues 3′ to the initiation codon. The second codon can influence the rate of initiation, with the magnitude depending on the initiation codon. The data are consistent with a simple kinetic model in which a variety of rate constants contribute to the process of translation initiation.