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Z‐DNA as a probe for localized supercoiling in vivo
Author(s) -
Rahmouni A. Rachid
Publication year - 1992
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1992.tb01502.x
Subject(s) - dna supercoil , biology , dna , escherichia coli , biophysics , transcription (linguistics) , in vivo , genetics , gene , dna replication , linguistics , philosophy
Summary Biological processes such as transcription are expected to generate local variations in DNA super‐coiling. The existence of localized supercoiling was recently demonstrated in Escherichia coli by using the supercoil‐driven B‐to‐Z transition as a super‐helicity probe. This new methodology is described and its extension to other biological systems discussed.