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Efficient repression by a heterodimeric repressor in Escherichia coli
Author(s) -
Webster C.,
Merryweather A.,
Brammar W.
Publication year - 1992
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1992.tb01480.x
Subject(s) - repressor , operator (biology) , biology , psychological repression , escherichia coli , lac repressor , binding site , yy1 , genetics , gene , gene expression , promoter
Summary Previous studies with purified variants of the 434 repressor having different operator‐binding specificities have demonstrated the interactions of a heterodimeric repressor with a hybrid operator site. The present study investigates the interactions between the 434 repressor and its operator site. The optimum 434 operator half‐site is used with a P22 operator half‐site to create a hybrid 434/P22 operator. We show that this hybrid operator can be efficiently bound by a heterodimeric repressor, consisting of one wild‐type 434 repressor monomer and one 434 repressor monomer with the binding specificity of the P22 repressor, to bring about repression in Escherichia coli.