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Biological roles of the Escherichia coli RuvA, RuvB and RuvC proteins revealed
Author(s) -
West Stephen C.,
Connolly Bernadette
Publication year - 1992
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1992.tb01454.x
Subject(s) - holliday junction , branch migration , biology , genetic recombination , dna , heteroduplex , dna repair , recombination , escherichia coli , microbiology and biotechnology , homologous recombination , genetics , gene
Summary In Escherichia coli , the ruvA, ruvB and ruvC gene products are required for genetic recombination and the recombinational repair of DNA damage. New studies suggest that these three proteins function late in recombination and process Holliday junctions made by RecA protein‐mediated strand exchange. In vitro , RuvA protein binds a Holliday junction with high affinity and, together with RuvB (an ATPase), promotes ATP‐dependent branch migration of the junction leading to the formation of heteroduplex DNA. The third protein, RuvC, which acts independently of RuvA and RuvB, resolves recombination intermediates by specific endonucleolytic cleavage of the Holliday junction.