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Identification and sequence of a Na + ‐linked gene from the marine bacterium Alteromonas haloplanktis which functionally complements the dagA gene of Escherichia coli
Author(s) -
MacLeod Patricia R.,
MacLeod Robert A.
Publication year - 1992
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1992.tb01444.x
Subject(s) - biology , permease , alteromonas , escherichia coli , biochemistry , complementation , plasmid , peptide sequence , enterobacteriaceae , alanine , gene , microbiology and biotechnology , amino acid , bacteria , genetics , phenotype
Summary A 4.0 kb fragment from a plasmid genomic DNA library of the marine bacterium Alteromonas haloplanktis ATCC 19855 was found in the presence of Na + to complement the dagA gene of Escherichia coli. We have completely sequenced this fragment and the position of the Na + ‐linked D‐alanine glycine permease gene (dagA) on the fragment has been determined by complementation. The predicted carrier protein consists of 542 amino acid residues (M r 58955). Its hydropathy profile suggests it is composed of eight transmembrane segments with a long hydrophilic region between segments six and seven. Significant similarity has been found between this Na + ‐linked permease and the Na*/proline permeases of E. coli and Salmonella typhimurium and the human and rabbit intestinal Na + /glucose cotransporters.