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Expression of bacterial cytotoxin genes in mammalian target cells
Author(s) -
Wels W.,
Baldrich M.,
Chakraborty T.,
Gross R.,
Goebel W.
Publication year - 1992
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1992.tb01442.x
Subject(s) - biology , bordetella pertussis , pseudomonas exotoxin , gene , adp ribosylation , toxin , exotoxin , adenylate kinase , cytotoxic t cell , pertussis toxin , cyclase , protein subunit , microbiology and biotechnology , bacteria , g protein , biochemistry , in vitro , recombinant dna , receptor , genetics , enzyme , nad+ kinase
Summary We have studied the expression of the gene fragments encoding the enzymatically active portion of three bacterial cytotoxins: exotoxin A (ETA) of Pseudomonas aeruginosa , and pertussis toxin (PT) and adenylate cyclase toxin (CYA) of Bordetella pertussis , in sensitive mammalian target cells. Expression of active ETA and CYA was lethal to the producing cells and stable transfectants of Cos‐1 cells containing the corresponding genes could not be obtained. The expression of the PTS1 subunit was tolerated by the producing mammalian cells. Since PT is cytotoxic because of ADP‐ribosylation of G‐proteins, we assume that the endogenously expressed PTS1 may not find the cellular target G proteins or PTS1 alone may not be sufficient for ADP‐ribosylation of these proteins in vivo.