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Clarification of the structural and functional features of the osmoregulated kdp operon of Escherichia coli
Author(s) -
Sugiura A.,
Nakashima K.,
Tanaka K.,
Mizuno T.
Publication year - 1992
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1992.tb01349.x
Subject(s) - biology , operon , escherichia coli , escherichia coli proteins , enterobacteriaceae , computational biology , microbiology and biotechnology , biochemistry , gene
Summary Expression of the Escherichia coli kdpABC operon, which is responsible for a high‐affinity potassium‐uptake system, is regulated in response to a change in the medium osmolarity. In this study, we clarified the structure and function of the kdpABC promoter including its regulatory sequence at the molecular level. The canonical ‐35 and ‐10 regions determined for the promoter were not fully functional, i.e. in addition to them, a cis ‐acting sequence located upstream of the ‐35 region was essential for full activation of the promoter. This upstream sequence was demonstrated to be the target site for the trans ‐acting activator, KdpE.