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Escherichia coli integration host factor stabilizes bacteriophage Mu repressor interactions with operator DNA in vitro
Author(s) -
Alazard R.,
Bétermier M.,
Chandler M.
Publication year - 1992
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1992.tb00895.x
Subject(s) - lysogenic cycle , biology , lytic cycle , repressor , bacteriophage , escherichia coli , operator (biology) , dna , host factor , genetics , in vitro , microbiology and biotechnology , gene , transcription factor , virus
Summary Using gel retardation and DNase I protection techniques, we have demonstrated that the Escherichia coli integration host factor (IHF) stabilizes the interaction between Mu repressor and its cognate operator‐binding sites in vitro. These results are discussed in terms of a model in which IHF may commit the phage to the lytic or lysogenic pathway depending on the occupancy of the operator sites by the repressor.