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Phylogeny of LCR‐1 and OXA‐5 with class A and class D β‐lactamases
Author(s) -
Couture France,
Lachapelle Jean,
Levesque Roger C.
Publication year - 1992
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1992.tb00894.x
Subject(s) - biology , phylogenetics , amino acid , gene , genetics , sequence alignment , class (philosophy) , peptide sequence , enzyme , nucleic acid sequence , computational biology , biochemistry , evolutionary biology , artificial intelligence , computer science
Summary The nucleotide sequences of bla LCR‐1 and bla OXA‐5 β‐lactamase genes have been determined. Polypeptide products of 260 and 267 amino acids with estimated molecular masses of 27 120 Da and 27387 Da were obtained for the mature form of LCR‐1 and OXA‐5 proteins. A progressive alignment was used to evaluate the extent of identity between LCR‐1 and OXA‐5 with 29 other β‐lactamase amino acid sequences. The data showed that both belong to class D. We identified amino acids conserved in 24 positions for class A β‐lactamases and in 28 positions for five class D enzymes. The structural similarities between class A and class D β‐lactamases are more extensive than indicated by earlier biochemical studies with overall 16% identity between both classes. From the alignment, dendograms were constructed with a distance‐matrix and parsimony methods which defined three major groups of proteins subdivided into clusters giving insight on β‐lactamase phylogeny and evolution.