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petR , located upstream of the fbcFBC operon encoding the cytochrome bc 1 complex, is homologous to bacterial response regulators and necessary for photosynthetic and respiratory growth of Rhodobacter capsulatus
Author(s) -
Tokito Mariko K.,
Daldal Fevzi
Publication year - 1992
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1992.tb00889.x
Subject(s) - operon , biology , rhodobacter , mutant , cytochrome , gene , nucleic acid sequence , genetics , mutagenesis , microbiology and biotechnology , biochemistry , enzyme
Summary Interposon mutagenesis of a region upstream of the petABC(fbcFBC) operon, encoding the ubiquinol: cytochrome c 2 oxidoreductase (bc 1 complex) of the photosynthetic bacterium Rhodobacter capsulatus revealed the presence of two genes, petP and petR. DNA nucleotide sequence determination of this region indicated that petP and petR are transcribed In the same direction as the petABC(fbcFBC) operon, and are translationally coupled. A silent insertion located In the interoperonal region separating petPR and the petABC(fbcFBC) genes indicated that these clusters have separate promoters. The deduced amino acid sequence of the putative petR gene product is homologous to various bacterial response regulators, especially to those of the OmpR subgroup. Moreover, it was found that PetR ‐ mutants are unable to grow on rich or minimal media by either photosynthesis or respiration, demonstrating that these gene products are essential for growth of R. capsulatus.