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Sequencing, mutational analysis, and transcriptional regulation of the Escherichia coli htrB gene
Author(s) -
Karow M.,
Georgopoulos C.
Publication year - 1991
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1991.tb02159.x
Subject(s) - biology , open reading frame , gene , escherichia coli , genetics , gene expression , regulation of gene expression , microbiology and biotechnology , northern blot , dna , peptide sequence
Summary The Escherichia coli htrB gene was originally discovered because its insertional inactivation led to an exquisitely temperature‐sensitive phenotype in rich media, i.e. the ability to form colonies at temperatures below 32°C, but not above 33°C. The htrB gene has been sequenced. It can potentially code for two proteins, with M r values of 35407 Da and 8669 Da, that are encoded by overlapping, divergent open reading frames. Our data are consistent with the 35407 Da protein being HtrB. Northern blot analysis clearly shows that the monocistronic htrB message is not under heat‐shock regulation. We have also sequenced the flanking DNA and have discovered a new gene, designated orf39.9 , located immediately adjacent to htrB , but divergently transcribed.