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Cloning of a sequence of Aquaspirillum magnetotacticum that complements the aroD gene of Escherichia coli
Author(s) -
Berson A. E.,
Hudson D. V.,
Waleh N. S.
Publication year - 1991
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1991.tb02156.x
Subject(s) - biology , cosmid , escherichia coli , complementation , genetics , homology (biology) , gene , microbiology and biotechnology , biochemistry , phenotype
Summary A 2 kb DNA fragment isolated from a cosmid library of Aquaspirillum magnetotacticum strain MS‐1 complements the aromatic‐metabolite requirements and iron‐uptake deficiencies of Escherichia coli and Salmonella typhimurium strains that lack a functional aroD (biosynthetic dehydrodquinase) sequence. All recombinant cosmids selected for their aroD complementation property carry this sequence. No DNA sequence homology has, however, been detected by Southern hybridization between the cloned fragment and the aroD gene of E. coli or the qa2 (catabolic dehydroquinase) gene of Neurospora crassa.