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Design of camp–CRP‐activated promoters in Escherichia coli
Author(s) -
ValentinHansen P.,
Hoist B.,
SøgaardAndersen L.,
Martinussen J.,
Nesvera J.,
Douthwaite S. R.
Publication year - 1991
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1991.tb02126.x
Subject(s) - biology , escherichia coli , promoter , escherichia coli proteins , genetics , microbiology and biotechnology , computational biology , gene , gene expression
Summary We have studied the doeP2 promoter of Escherichia coli to define features that are required for optimal activation by the complex of adenosine 3′, 5′ monophosphate (cAMP) and the cAMP receptor protein (CRP). Systematic mutagenesis of deoP2 shows that the distance between the CRP site and the ‐10 hexamer is the crucial factor in determining whether the promoter is activated by camp–CRP. Based on these observations, we propose that camp–CRP‐activated promoters can be created by correctly aligning a CRP target and a ‐ 10 hexamer. This idea has been successfully tested by converting both a CRP‐in‐dependent promoter and a sequence resembling the consensus ‐10 hexamer to strongly camp–CRP‐activated promoters.