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deoP1 promoter and operator mutants in Escherichia coli : isolation and characterization
Author(s) -
Dandanel G.,
Hammer K.
Publication year - 1991
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1991.tb02083.x
Subject(s) - biology , operon , rna polymerase , genetics , operator (biology) , repressor , mutant , palindromic sequence , promoter , base pair , dna , plasmid , consensus sequence , microbiology and biotechnology , upstream activating sequence , escherichia coli , palindrome , gene , base sequence , crispr , transcription factor , gene expression
Summary Plasmid DNA containing deoP1 , one of the two major promoters of the deo operon, has been mutagenized using hydroxylamine, and promoter down‐mutations and operator mutations were selected. The isolated mutants are all located within a 16bp palindromic sequence containing the –10 region of deoP1. The results show that RNA polymerase and DeoR repressor compete for the same DNA target. The deoP1 promoter activity is dependent on a TG motif one base pair upstream of the –10 consensus sequence. The sequence of the deo operator site was further verified by use of a synthetic linker.