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DNA sequencing and complementation/deletion analysis of the bchA‐puf operon region of Rhodobacter sphaeroides: in vivo mapping of the oxygen‐regulated puf promoter
Author(s) -
Hunter C. N.,
McGlynn P.,
Ashby M. K.,
Burgess J. G.,
Olsen J. D.
Publication year - 1991
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1991.tb01974.x
Subject(s) - operon , biology , complementation , rhodobacter sphaeroides , open reading frame , gene , genetics , rhodobacter , l arabinose operon , gal operon , plasmid , microbiology and biotechnology , peptide sequence , mutant , bacteria
Summary Within the photosynthetic gene cluster of Rhodobacter sphaeroides the genes encoding light‐harvesting LHI and reaction‐centre complexes are transcriptionally linked in the order pufBALMX. The region stretching 1.6 kb upstream of pufB has been examined by DNA sequencing and by complementation/deletion analysis. These studies demonstrate that three open reading frames are located upstream of pufB. One open reading frame, designated bchA , terminates just Inside pufQ , which is located proximal to pufB. BchA contains a 37 bp region that functions as the oxygen‐regulated promoter for pufQ , and probably for the puf operon as a whole. We also demonstrate that the protein encoded by pufQ appears to play a role in bacteriochlorophyll biosynthesis.