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Both genes for EF‐1α in Candida albicans are translated
Author(s) -
Sundstrom P.,
Irwin M.,
Smith D.,
Sypherd P. S.
Publication year - 1991
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1991.tb01918.x
Subject(s) - biology , candida albicans , gene , microbiology and biotechnology , computational biology , genetics
Summary In previous work, we showed that Candida albicans has two genes, TEF‐1 and TEF‐2 , which encode identical polypeptides for the highly conserved, essential, protein synthesis factor EF‐1α (Breviario et al. , 1988). This result prompted questions as to whether C. albicans preferentially uses one of the genes over the other and whether both genes are actually translated into protein. Gene‐specific sequence differences in the untranslated portion of each gene made it possible to prepare gene‐specific oligonucleotide hybridization probes. Results with the probes showed that the relative steady‐state mRNA levels of the two genes were equivalent and that the mRNA for each gene was present in active translation complexes.