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The repair of double‐strand breaks and S1 nuclease‐sensitive sites can be monitored chromosome‐specifically in Saccharomyces cerevisiae using pulsed‐field gel electrophoresis
Author(s) -
Geigl E.M.,
EckardtSchupp F.
Publication year - 1991
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1991.tb01908.x
Subject(s) - biology , saccharomyces cerevisiae , nuclease , dna , chromosome , ploidy , yeast , gel electrophoresis , dna repair , microbiology and biotechnology , dna damage , genetics , gene
Summary Repair under non‐growth conditions of DNA double‐stranded breaks (DSBs) and S1 nuclease‐sensitive sites (SSSs; e.g. DNA damage which is processed by in vitro treatment with S1 nuclease to DSBs) induced by [ 60 Co]‐gamma‐rays (200 Gy; anoxic conditions) was monitored in a diploid repair‐competent strain of Saccharomyces cerevisiae. We used pulsed‐field gel electrophoresis (PFGE), which allows the separation of chromosome‐sized yeast DNA molecules, to determine the number of DSBs and SSSs in Individual chromosome species of yeast. Our results indicate that SSSs which have been regarded as clusters of base damage in opposite DNA strands are repaired efficiently in a repair‐proficient diploid strain of yeast. The time course of SSS repair is comparable to the one of DSB repair, indicating similarities in the molecular mechanism. Both types of repair kinetics are different for different chromosome species.