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Genetic and biochemical analysis of the aspartokinase from Corynebacterium glutamicum
Author(s) -
Kalinowski J.,
Cremer. J.,
Bachmann B.,
Eggeling L.,
Sahm H.,
Pühler A.
Publication year - 1991
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1991.tb01893.x
Subject(s) - corynebacterium glutamicum , biology , locus (genetics) , gene , amino acid , mutant , genetics , expression vector , coding region , microbiology and biotechnology , recombinant dna
Summary The lysC/asd gene cluster of Corynebacterium glutamicum ATCC 13032 was cloned and sequenced. The lysC locus coding for aspartokinase consists of two in‐frame overlapping genes, lysCα encoding a protein of 421 amino acids ( M r 44300) and lysCβ encoding a protein of 172 amino acids ( M r 18600). The C. glutamicum aspartokinase was purified and found to contain two proteins of M r 47000 and M r 18000. A C. glutamicum mutant expressing a feedback‐resistant aspartokinase was shown to be changed in a single base pair of the lysC β gene, leading to an amino acid exchange in the β‐subunit of the aspartokinase. In addition, the identified mutation was found to be responsible for the enhanced expression of the asd gene located downstream of lysC.