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A cytosine‐ over guanosine‐rich sequence in RNA activates rho‐dependent transcription termination
Author(s) -
Rivellini F.,
Alifano P.,
Piscitelli C.,
Blasi V.,
Brunt C. B.,
Carlomagno M. S.
Publication year - 1991
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1991.tb01864.x
Subject(s) - biology , guanosine , cytosine , transcription (linguistics) , dna , operon , rna , genetics , microbiology and biotechnology , escherichia coli , gene , linguistics , philosophy
Summary We have constructed an expression vector carrying the Escherichia coli his operon control region to study the ability of defined segments of DNA to cause rho tactor‐mediated transcription termination both in vivo and in vitro. We have previously identified a consensus motif consisting of a region of high cytosine over guanosine content common to several cryptic intracistronic transcription termination elements unmasked by polar mutations. We show that a DNA fragment possessing features similar to the ones previously identified is capable of causing rho‐mediated release of transcripts in vivo and in vitro. The efficiency of termination depends on the length and relative cytosine over guanosine ratio of the element.