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Expression of the gene encoding glycerol‐3‐phosphate dehydrogenase ( glpD ) in Bacillus subtilis is controlled by antitermination
Author(s) -
Holmberg C.,
Rutberg B.
Publication year - 1991
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1991.tb01849.x
Subject(s) - biology , bacillus subtilis , gene , transcription (linguistics) , gene expression , microbiology and biotechnology , antitermination , sigma factor , psychological repression , rna polymerase , regulation of gene expression , reporter gene , dehydrogenase , promoter , biochemistry , genetics , rna , enzyme , bacteria , philosophy , linguistics
Summary The Bacillus subtilis glpD gene encodes glycerol‐3‐phosphate (G3P) dehydrogenase. A sigma A type promoter and the transcriptional startpoint for glpD were identified. Between the transcriptional start‐point and glpD there is an inverted repeat followed by a run of T residues. The inverted repeat prevents expression of a reporter gene, xylE , when positioned between this gene and a constitutive promoter. Expression of xylE , like expression of glpD , is induced by G3P and repressed by glucose. Induction also requires the product of the glpP gene. Our results suggest that glpD expression is controlled by antitermination of transcription. The inverted repeat appears to be a target for induction by G3P and GlpP. We speculate that glucose repression is mediated via an inhibitory effect on synthesis or activity of GlpP.