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Transcription of the Vibrio cholerae haemolysin gene, hlyA , and cloning of a positive regulatory locus, hlyU
Author(s) -
Williams S. G.,
Manning P. A.
Publication year - 1991
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1991.tb00825.x
Subject(s) - vibrio cholerae , biology , gene , locus (genetics) , genetics , hemolysin , cloning (programming) , transcription (linguistics) , molecular cloning , vibrio , vibrionaceae , microbiology and biotechnology , bacteria , gene expression , virulence , linguistics , philosophy , computer science , programming language
Summary Transcription of the Vibrio cholerae hlyA gene, which encodes a cytotoxic haemolysin, has been investigated. The hlyA transcript initiates 430 nucleotides (nt) upstream of the translational start site, hlyA – cat transcriptional fusion constructs were active in V. cholerae but not in Escherichia coli. An hlyA – cat fusion was used to select, from a V. cholerae O17 plasmid library, a clone that could activate the hlyA promoter in E coli. This regulatory locus has been designated hlyU. hlyU appears to be distinct from the previously described hlyR locus.