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A Bacillus subtilis dipeptide transport system expressed early during sporulation
Author(s) -
Mathiopoulos C.,
Mueller J. P.,
Slack F. J.,
Murphy C. G.,
Patankar S.,
Bukusoglu G.,
Sonenshein A. L.
Publication year - 1991
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1991.tb00814.x
Subject(s) - operon , bacillus subtilis , biology , dipeptide , auxotrophy , escherichia coli , biochemistry , permease , trp operon , gene , genetics , amino acid , bacteria
Summary Two previously identified Bacillus subtilis DNA segments, dciA and dciB , whose transcripts accumulate very rapidly after induction of sporulation, were found in the same 6.2 kb transcription unit, now known as the dciA operon. Analysis of the sequence of the dciA operon showed that its putative products are homologous to bacterial peptide transport systems. The product of the fifth gene, DciAE, is similar to peptide‐binding proteins from Escherichia coli and Salmonella typhimurium (DppA and OppA) and B. subtilis (OppA). A null mutation in dciAE abolished the ability of a proline auxotroph to grow in a medium containing the dipeptide Pro–Gly as sole proline source, suggesting that the dciA operon encodes a dipeptide transport system.