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Transformation of Mycobacterium aurum and Mycobacterium smegmatis with the broad host‐range Gram‐negative cosmid vector pJRD215
Author(s) -
Hermans J.,
Martin C.,
Huijberts G. N. M.,
Goosen T.,
Bont J. A. M.
Publication year - 1991
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1991.tb00803.x
Subject(s) - mycobacterium smegmatis , biology , cosmid , microbiology and biotechnology , kanamycin , transformation (genetics) , plasmid , mycobacterium , genetics , bacteria , mycobacterium tuberculosis , gene , antibiotics , medicine , tuberculosis , pathology
Summary The transformation of Mycobacterium aurum and Mycobacterium smegmatis with the Gram‐negative RSF1010‐derived cosmid pJRD215 is described. The plasmid is stably maintained in both species and the antibiotic resistance determinants for kanamycin and streptomycin are expressed. Southern blot analysis shows that rearrangements take place both in M. aurum and in M. smegmatis. The use of pJRD215 in mycobacterial cloning systems is discussed.