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Nucleotide sequence and genetic organization of the ferric enterobactin transport system: homology to other peripiasmic binding protein‐dependent systems in Escherichia coli
Author(s) -
Shea C. M.,
McIntosh M. A.
Publication year - 1991
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1991.tb00788.x
Subject(s) - enterobactin , biology , operon , escherichia coli , periplasmic space , siderophore , homology (biology) , peptide sequence , gene , bacterial outer membrane , nucleic acid sequence , biochemistry , membrane transport protein , genetics , transporter
Summary The nucleotide sequence of the Escherichia coli fep genomic region has been determined. Three new loci were identified. One of these, P43 , encodes a membrane protein that is not essential for ferric enterobactin transport. Two others, fepD and fepG , were found to be essential for transport and their translational products showed extensive homology to other integral membrane proteins involved in TonB‐dependent transport processes. The FepC amino acid sequence suggested a peripheral membrane location and revealed conserved ATP‐binding domains. Together these data indicate that ferric enterobactin is transported through a typical periplasmic binding protein‐dependent system, in addition, the transcriptional organization of these genes was examined and primer extension analysis identified a single iron‐regulated bidirectional promoter between the P43 gene and the fepDGC operon.